|
KMID : 1094720100150040603
|
|
Biotechnology and Bioprocess Engineering
2010 Volume.15 No. 4 p.603 ~ p.607
|
|
|
Development of an amphiphilic matrix for immobilization of Candida antartica lipase B for biodiesel production
|
|
Lee Ko-Woon
Min Kyoung-Seon
Park Kyung-Moon
Yoo Young-Je
|
|
|
Abstract
|
|
|
|
Biodiesel has been greatly interested as an alternative fuel and is produced by a transesterification reaction of oil with alcohol. Recently, microbial lipases have been used for biodiesel production. Among the microbial lipase, immobilized Candida antartica lipase B (CALB) is the most widely used. However, CALB is unstable and shows low catalytic efficiency in the reaction media because the reaction media contains a high concentration of methanol and the lipase is also inhibited by the by-product glycerol. In this study, to overcome these limitations, we developed an amphiphilic matrix to immobilize CALB. The immobilized lipase in an amphiphilic matrix with 80% ethyltrimethoxysilane (ETMS) in tetramethoxysilane (TMOS) and pretreated with oil showed the highest specific activity and biodiesel conversion ratio; about 90% biodiesel conversion in 24 h at an initial molar ratio of 1: 1 (oil: methanol) with stepwise methanol feeding in order to adjust the net molar ratio to be 1: 3.
|
|
|
KEYWORD
|
|
|
amphiphilic matrix, Candida antartica lipase B (CALB), immobilization, biodiesel production
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|